Bone Decalcification Methods
- Bone decalcification is used to study calcium-rich bones.
- Scientists use bone decalcification to examine cartilage and magnesium levels.
- There are two categories of decalcifying agents: chelating agents and acids.
- The most frequently used chelating agent is Ethylenediaminetetraacetic acid (EDTA).
- Decalcification is a lengthy procedure that requires leaving bone pieces in the decalcifying agent for days to weeks.
- X-ray examination is one method to test when bone decalcification is complete.
- Chemical analysis can also be used to determine the completion of decalcification.
- Measurement of specimen flexibility is another method to assess decalcification completion.
- Decalcification is necessary to obtain soft sections of the bone using a microtome.
- Thin sections of the bone can be processed like any other soft tissue of the body.
Bone Seeker
- Bone seeker is a term related to bone decalcification.
- Further information about bone seekers can be found in related sources.
References
- Howe, Percy (1922). Decalcification of teeth and bones, and regeneration of bone through diet.
- Prasad, Donoghue (2013). A comparative study of various decalcification techniques.
- Hutchison, Florence (1992). Osteomalacia and rickets.
- Callis, Gyle (1998-03-01). Decalcification of bone: literature review and practical study of various decalcifying agents.
- Authority control databases: National Czech Republic. Retrieved from https://en.wikipedia.org/w/index.php?title=Bone_decalcification&oldid=1182028492
Categories
- Anatomical pathology
- Bones
- Calcium
- Hidden category: Articles with NKC identifiers
Bone decalcification is the softening of bones due to the removal of calcium ions, and can be performed as a histological technique to study bones and extract DNA. This process also occurs naturally during bone development and growth, and when uninhibited, can cause diseases such as osteomalacia.